Low-endotoxin Glycine Buffer is commonly employed in a variety of laboratory techniques, including use as a running buffer for polyacrylamide gel electrophoresis, a transfer buffer in Western blotting, enzyme-based assays, and selected cell culture applications. This 0.2 M formulation, adjusted to pH 3.0, provides reliable buffering capacity within the acidic pH range of approximately 2.2 to 3.6, supporting stable experimental conditions. Glycine buffer plays a critical role in numerous protein purification workflows, particularly in the isolation and recovery of monoclonal antibodies.