Boston BioProducts' Buffer Calculator
Biological buffers play a key role in maintaining the pH and environmental conditions of scientific processes and ensure controlled experiments with preserved results.
The concentration of a buffer's raw materials holds a direct impact in its final application and must have correct measurements.
This calculator is a useful tool to compute buffer concentration in molarity (M, mM, or nM) and prepare buffer solutions using the molar mass of the raw material selected and the desired final volume (L, mL, or μL).
How to use the Buffer Calculator:
- Select your desired raw material from the dropdown.
- Insert the desired final volume and concentration of your buffer into the corresponding fields.
- Click "Calculate". The correct mass of your desired raw material will instantly populate below.
Reference Formulas
Henderson-Hasselbach equation:
pH = pKa + log([A⁻]/[HA])
Percentage by weight (w/v) =
(g solute / 100g of solution) × 100
Mass by concentration =
Desired concentration × molar mass of raw material × final volume
Buffer Parameters
CAS #:-
pKa:-
Buffer pH Range:-
Formula Weight (g/mol):-
0.00g
Mass: