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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Lab technician handling qPCR buffer reagents at a workbench
  1. Home
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  4. qPCR Buffers

qPCR Buffers

A qPCR buffer is formulated to ensure optimal amplification efficiency, sensitivity, and reproducibility in quantitative PCR workflows. It creates the precise chemical environment needed for robust DNA polymerase activity and accurate quantification of target sequences—even in low-abundance or complex samples.

  • Provides consistent pH, salt concentration, and cofactor balance for high-performance amplification
  • Enhances primer binding and enzyme stability throughout thermal cycling
  • Compatible with both DNA and RNA targets in SYBR Green, probe-based, and multiplex qPCR assays

Whether you're performing gene expression profiling, SNP genotyping, or viral load quantification, our qPCR buffer products deliver the precision your results depend on.

qPCR Buffers

Showing 1–11 of 11 products

RNA Storage Buffer (1 mM Sodium Citrate, pH 6.4)

RNA Storage Buffer (1 mM Sodium Citrate, pH 6.4)

From: $68.00

250ml

Plasmid DNA Elution Buffer II

From: $46.00

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Elution Buffer (pH 8.5)

From: $46.00

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Wash Buffer (pH 7.0)

From: $49.00

Plasmid DNA Isolation Buffer (pH 7.0)

Plasmid DNA Isolation Buffer (pH 7.0)

From: $51.00

Plasmid DNA Lysis Solution (pH > 11.0)

Plasmid DNA Lysis Solution (pH > 11.0)

From: $51.00

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

From: $42.00

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Resuspension Buffer (pH 8.0)

From: $42.00

PCR Amplification Buffer (10X)

PCR Amplification Buffer (10X)

From: $250.00

qPCR DNase Buffer (10X)

qPCR DNase Buffer (10X)

From: $126.00

qPCR Proteinase K Buffer (10X)

qPCR Proteinase K Buffer (10X)

From: $63.00

FAQs

A qPCR buffer supports the amplification and detection of target DNA or RNA in real-time PCR by maintaining optimal pH, salt levels, and cofactor availability for the DNA polymerase.

Typical components include Tris (for pH control), KCl or MgCl₂ (to support enzyme activity), and additives like DMSO or glycerol to stabilize the reaction and improve amplification of difficult templates.

Absolutely. pH, magnesium concentration, and additives can significantly impact amplification efficiency, specificity, and sensitivity, especially in low-copy or GC-rich targets.