Blood Lysis Buffer

Blood cell lysis is the breaking or disruption of blood cells (red blood cells called erythrocytes and white blood cells called leukocytes). Scientists typically employ mechanical or non-mechanical methods to lyse cells to extract proteins for downstream experiments.

A blood lysis buffer disrupts the blood cell's membrane by altering pH. A range of buffers can lyse red or white blood cells. If detergents are added to buffers, the release of soluble proteins is accelerated, and a purification step must be added to the cell lysis protocol.