This low-endotoxin glycine buffer is formulated to provide consistent pH control under strongly acidic conditions, with effective buffering between pH 2.2 and 3.6. At 0.2 M and pH 2.5, it is widely used in electrophoresis workflows, including as a running buffer and for protein transfer during Western blotting. The buffer is also suitable for enzyme assays, selected cell culture applications, and downstream bioprocessing steps. Glycine buffers are frequently employed in protein purification protocols, particularly for elution and recovery of monoclonal antibodies where low endotoxin levels are required.