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Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
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Tris-Glycine-SDS Running Buffer (10X)
The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.
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Transfer Buffer (10X, Electro Blotting)
The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.
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EDTA & EGTA Solutions | Boston BioProducts

Researcher preparing EDTA and EGTA chelating solutions in a lab

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EDTA & EGTA

EDTA & EGTA

EDTA and EGTA solutions are chelating agents that bind divalent metal ions to inhibit metal-dependent enzymes or prevent unwanted reactions.

Chelate Ca²⁺ and Mg²⁺ ions
Protect proteins and nucleic acids from metal-dependent degradation
Available in aqueous buffer formulations
Useful in cell lysis and enzyme inhibition

Commonly used in cell lysis, protein purification, and reactions sensitive to divalent cations.

EDTA & EGTA

Showing 1–9 of 9 products

EDTA (0.5 M, pH 7.4, pH Adjusted with KOH)

EDTA (0.5 M, pH 7.4, pH Adjusted with KOH)

From: $57.00

EDTA (0.5 M, pH 8.0, pH Adjusted with KOH)

EDTA (0.5 M, pH 8.0, pH Adjusted with KOH)

From: $37.00

EDTA (0.5 M, pH 7.4)

EDTA (0.5 M, pH 7.4)

From: $31.00

EGTA (0.5 M, pH 8.0)

EGTA (0.5 M, pH 8.0)

From: $51.00

EGTA (0.5 M, pH 7.4)

EGTA (0.5 M, pH 7.4)

From: $89.00

EDTA (0.5 M, pH 8.0)

EDTA (0.5 M, pH 8.0)

From: $31.00

EGTA (1 M, pH 8.0)

EGTA (1 M, pH 8.0)

From: $159.00

EGTA (1 M, pH 7.4)

EGTA (1 M, pH 7.4)

From: $89.00

EDTA (0.5 M, pH 8.0, RNase Free)

EDTA (0.5 M, pH 8.0, RNase Free)

From: $34.00

FAQs

Products & Formulation

EDTA binds a broad range of divalent cations; EGTA preferentially chelates Ca²⁺.

Yes, but only at concentrations that do not disrupt essential metal-dependent processes.