What is glycine buffer used for?

Glycine buffer is commonly used in western blotting, protein purification, and as an elution buffer for monoclonal antibody purification.

Can glycine buffer interfere with protein activity?

Glycine is generally non-denaturing, but its low-pH formulations may temporarily alter protein conformation; neutralization is recommended post-elution.

What is the purpose of glycine in a running buffer system?

Glycine serves as a buffering ion and trailing ion in electrophoresis systems, helping to maintain pH and regulate the movement of charged molecules through the gel during separation.

Glycine Buffer

Glycine buffer is an amino acid-based solution commonly used to maintain pH in the range of 2.0 to 3.0 or 8.0 to 10.0, depending on formulation. It’s frequently used in biochemical and immunological applications where precise pH control is essential.

Supports protein transfer and blocking steps in western blotting
Ideal for purification and recovery of monoclonal antibodies
Ready-to-use formulation eliminates variability and improves lab efficiency

Whether you're working on protein purification, blotting techniques, or pH-dependent assays, our glycine buffer delivers dependable performance with every batch.