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Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
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Tris-Glycine-SDS Running Buffer (10X)
The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.
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Transfer Buffer (10X, Electro Blotting)
The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.
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News

Nirrin Technologies and Boston BioProducts Raise the Bar on Quality Excellence in GMP Buffer Manufacturing

October 22, 2024

Nirrin Technologies and Boston BioProducts Raise the Bar on Quality Excellence in GMP Buffer Manufacturing

Boston BioProducts adopts Nirrin’s Atlas system to enhance Quality Control (QC) testing and set new quality standards

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Recent Updates

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Boston BioProducts Bolsters Commercial Leadership Team with New Head of Product Management

NewsJuly 9, 2024

Boston BioProducts Bolsters Commercial Leadership Team with New Head of Product Management

Mahadevan Sethuraman Joins to Drive Growth Across Cell Culture Reagents, Diagnostic Tools, and Bioprocessing Solutions

Boston BioProducts Strengthens Leadership Team, Setting New Standards in Reagent Manufacturing and Quality

NewsNovember 9, 2023

Boston BioProducts Strengthens Leadership Team, Setting New Standards in Reagent Manufacturing and Quality

Strategic Hires Include Kammie McHugh as Director of Quality & Regulatory and Matt Smart as Director of Process Excellence

Boston BioProducts Receives ISO 13485:2016 Quality Certification

NewsApril 5, 2023

Boston BioProducts Receives ISO 13485:2016 Quality Certification

Receives ISO 13485:2016 Quality Certification

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