The Glycine Buffer in PBS is widely used in various laboratory applications such as polyacrylamide gel electrophoresis as a running buffer, a transfer buffer for Western blotting, enzyme assays, and cell culture. This Buffer type serves as a tool for maintaining stable conditions during biochemical experiments in the pH range of 2.2 to 3.6. The Glycine buffer is an essential part of many types of protein purification procedures, including the purification and recovery of monoclonal antibodies.