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Characteristics Tests:
Validated method: Measure a solution's unique absorbance wavelength.
USP <644> method: Measures the concentration and mobility of ions in a solution.
USP <841> method: Measures how tightly packed molecules are in a solution.
USP <785> method: Measures total concentration of solute particulates in a solution.
USP <791> method: Measures concentration of hydrogen ions (pH) in a liquid solution.
USP <831> method: Measures the velocity that light travels through a substance.
Customizable study: Measures a product's consistency and shelf life over a set storage and time period.
USP <914> method: Measures a liquid's resistance to flow.
Contamination Control Tests:
USP <61> method: Detect and quantify viable microorganisms in a product.
Validated method: Detects the presence of DNase enzyme activity.
USP <85> method: Measures levels of pyrogenic lipopolysaccharides (LPS) found in gram-negative bacteria.
Validated method: Fast turnaround alternative to USP <71> Sterility testing.
Validated method: Detects the presence of protease enzyme activity.
Validated method: Detects presence of RNase enzyme activity.
USP <71> method: Confirms absolute absence of microorganisms in sterile products.
Featured Products
Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Tris-Glycine-SDS Running Buffer (10X)
The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.
Transfer Buffer (10X, Electro Blotting)
The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.