This Glycine Buffer is prepared at a concentration of 0.2 M and adjusted to pH 3.0 to provide effective buffering across the acidic range of approximately pH 2.2 to 3.6. It is routinely used in molecular biology and biochemistry laboratories for electrophoresis systems, protein transfer applications, enzyme assays, and cell-based studies. Glycine buffer is a key component in many protein purification workflows, particularly those involving antibody elution and downstream processing.