
Luminol & Oxidizing Solutions are ready-to-use reagents designed for chemiluminescent detection in protein analysis, especially Western blotting. When mixed together, luminol and an oxidizing solution react with horseradish peroxidase (HRP)-labeled antibodies to produce a light signal that can be detected on film or imaging systems, enabling sensitive protein visualization.
Luminol & Oxidizing Solutions are commonly used in Western blotting and other immunodetection workflows where sensitive protein detection is required.
The luminol reagent reacts with an oxidizing agent in the presence of HRP-conjugated antibodies to emit light, allowing chemiluminescent detection of target proteins on membranes.
It’s used primarily in Western blotting and other HRP-based immunoassays to visualize proteins with a chemiluminescent signal.
Mix equal volumes of the luminol and oxidizing solutions just before use, then apply to the membrane after antibody incubation and washes.
