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Accelerate Upstream Bioprocessing

Improve cell line development, scale up cultures, and safeguard your workflow with ready-to-use catalog buffers and media, or customize formulations through our in-house services for your specific needs.

FAQs

Buffers maintain pH, osmotic balance, and nutrient stability, ensuring healthy cell growth and consistent recombinant protein or bioproduct expression.

It includes culturing cells, optimizing growth and density, inducing protein or antibody expression, and harvesting for downstream purification.

Proteins, monoclonal antibodies, DNA/RNA constructs, enzymes, and other bioproducts rely on optimized upstream bioprocessing conditions.

Prokaryotes (bacteria, archaea) and eukaryotes (yeast, plant, insect, mammalian cells) are used depending on the product and expression system.

Yes. Boston BioProducts offers ready-to-use and custom buffers tailored for cell type, growth conditions, and protein expression requirements.

Precise formulation and consistent quality reduce variability, support cell health, and maximize yield and reproducibility for downstream production.

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    Featured Products

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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