Product Description

The Separating (Resolving) Buffer is a critical component in SDS-Polyacrylamide Gel Electrophoresis (PAGE), a laboratory technique that is used to separate protein molecules based on molecular weight. This buffer maintains the pH of the separating or resolving gel at 8.8, which is a higher pH level than that of the stacking gel.

Specifications

Component Name	CAS	Molecular Weight	Concentration
Tris-Base / Tris-HCl	77-86-1 / 1185-53-1	121.14 / 157.60	1.5 M
Sodium Dodecyl Sulfate (SDS) [20% Solution]	151-21-3	288.38	0.40%
Water	7732-18-5	18.02	Rest

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Separating (Resolving) Buffer

Product Description

Specifications

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Separating (Resolving) Buffer

Separating (Resolving) Buffer

Separating (Resolving) Buffer

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Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

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Product Citations

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

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Need a specific formulation?

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

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Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Separating (Resolving) Buffer

Product Description

Specifications

Documents

Product Citations

Related Products

Related Resources

Need a specific formulation?

Separating (Resolving) Buffer

Separating (Resolving) Buffer

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Related Resources

Need a specific formulation?

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)