Citrate Phosphate Buffer, commonly referred to as McIlvaine Buffer, is formulated using citric acid and sodium phosphate dibasic to deliver effective buffering across a broad pH range of approximately 3.0 to 8.0. This pH 6.0 formulation is well suited for use in histological techniques, medical diagnostic assays, immunohistochemistry, and a variety of molecular biology applications. Buffer performance can be precisely optimized by adjusting the relative proportions of citrate and phosphate ions, allowing stable and reproducible conditions for sensitive biochemical and analytical procedures.