Gel Stacking & Resolving Buffers

Gel casting buffers are essential for forming the gel matrix used in electrophoretic separation of biomolecules. These buffers maintain consistent pH and ionic strength during gel polymerization, supporting reproducible migration and sharp resolution across applications like SDS-PAGE.

Supports consistent gel formation for reproducible band separation
Maintains ideal pH and conductivity during polymerization and electrophoresis
Compatible with a range of acrylamide gels

Our gel casting buffers deliver reliability from start to finish for polyacrylamide gel casting.

A gel running buffer is used during electrophoresis to carry electric current and maintain pH, allowing biomolecules like DNA, RNA, or proteins to migrate consistently through the gel.

Discoloration, cloudiness, or a drop in conductivity may indicate degradation or contamination. Also, poor band resolution or unexpected migration can signal an issue with the buffer.

Tris-Glycine is widely used for standard SDS-PAGE, while Tris-Tricine is better suited for resolving small proteins or peptides below ~10 kDa.

A gel running buffer is used during electrophoresis to carry electric current and maintain pH, allowing biomolecules like DNA, RNA, or proteins to migrate consistently through the gel.

Discoloration, cloudiness, or a drop in conductivity may indicate degradation or contamination. Also, poor band resolution or unexpected migration can signal an issue with the buffer.

Tris-Glycine is widely used for standard SDS-PAGE, while Tris-Tricine is better suited for resolving small proteins or peptides below ~10 kDa.

Gel Casting

Gel Casting

Stacking Buffer

Separating (Resolving) Buffer

FAQs

Gel Casting

Stacking Buffer

Separating (Resolving) Buffer

FAQs