Tween-20, also known as Polysorbase 20, is a gentle nonionic surfactant commonly used in biochemical and life science research. It effectively solubilizes membrane proteins and is vital for applications such as western blotting, ELISAs, and immunohistochemistry. By minimizing non-specific binding and background staining, Tween-20 ensures precise and reliable results in buffer formulations for washing, blocking, and stabilizing.