Tween-20 (Polysorbate 20) is a mild, nonionic surfactant widely used in biochemical and life science laboratories. Supplied as a 20% solution for convenient handling and dilution, it aids in the solubilization of membrane-associated proteins and supports consistent assay performance. Tween-20 is commonly incorporated into wash and blocking buffers for techniques such as western blotting, ELISA, and immunohistochemistry, where it helps reduce background noise and improve signal clarity while maintaining protein stability.