EDTA (Ethylenediaminetetraacetic acid) is a metal ion chelator routinely employed in laboratory protocols that require removal or sequestration of divalent cations. At pH 8.0, this 0.5 M solution provides effective binding of calcium and magnesium ions and is widely used in molecular biology workflows, including cell lysis and sample stabilization. EDTA is also a standard additive in blood collection, where it prevents coagulation by eliminating free calcium. As EDTA has limited solubility at lower pH, pH adjustment is essential for preparation of aqueous solutions.