EGTA solution [Ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid] is widely employed in molecular biology and protein biochemistry protocols that require selective chelation of divalent metal ions, particularly calcium. Due to its higher affinity for calcium compared to magnesium, EGTA is especially valuable in biological systems where calcium regulation is critical and in analytical applications involving calcium measurement in the presence of magnesium. This 1 M solution adjusted to pH 8.0 provides effective and reliable metal ion chelation for consistent experimental outcomes.