Separating (Resolving) Buffer | Boston BioProducts

Separating (Resolving) Buffer

CAT#: BP-90

Useful in biochemistry and molecular biology techniques
Can be used to prepare the resolving gels in SDS-PAGE protocols
Provided at 4X concentrate and is ready-to-use once diluted to a 1X working concentration

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Product Description

The Separating (Resolving) Buffer is a critical component in SDS-Polyacrylamide Gel Electrophoresis (PAGE), a laboratory technique that is used to separate protein molecules based on molecular weight. This buffer maintains the pH of the separating or resolving gel at 8.8, which is a higher pH level than that of the stacking gel.

Specifications

Component Name	CAS	Molecular Weight	Concentration
Tris-Base / Tris-HCl	77-86-1 / 1185-53-1	121.14 / 157.60	1.5 M
Sodium Dodecyl Sulfate (SDS) [20% Solution]	151-21-3	288.38	0.40%
Water	7732-18-5	18.02	Rest

Documents

Safety Data Sheets (SDS)Document request form

Need a specific formulation?

We can manufacture custom buffers to your exact specifications.

Custom Reagent Builder

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Need a specific formulation?

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

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Need a specific formulation?

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Stacking Buffer

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Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Related Resources

Need a specific formulation?

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Related Resources

Need a specific formulation?

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 3.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 4.0)

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 5.0)

Stacking Buffer

Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?

Separating (Resolving) Buffer

Need a Custom Formulation?

Need a Custom Formulation?