HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] is a highly effective Good’s buffer widely used in biochemical and molecular biology experiments. It provides excellent pH stability between 6.8 and 8.5, ensuring consistent conditions for processes near physiological pH. This buffer is well suited for applications such as cell culture, enzyme assays, protein purification, isoelectric focusing, bioprocessing, and live-cell imaging, where precise pH control is essential for reliable experimental results.