HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] is a reliable Good’s zwitterionic buffer commonly used in biochemical and molecular biology workflows. This 1 M solution, adjusted to pH 8.0, offers strong buffering capacity within the effective pH range of approximately 6.8–8.2, helping maintain stable and reproducible experimental conditions near physiological pH. It is well suited for applications including cell culture, enzyme activity studies, protein purification, isoelectric focusing, bioprocessing, and live-cell imaging, where consistent pH control is critical for optimal performance and data quality.