Plasmid DNA Isolation Buffer (pH 7.0) | Boston BioProducts

Plasmid DNA Isolation Buffer (pH 7.0)

CAT#: DK-204

Sterile-filtered pDNA isolation buffer, pH adjusted to 7.0
For isolation of plasmids in bacterial cell lysate, following neutralization
Isolation buffer used as part of plasmid prep workflow for efficient isolation of pDNA

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We manufacture to your exact specifications.

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Product Description

Plasmid DNA Isolation Buffer is a MOPS-buffered saline solution with Triton X-100 detergent and isopropanol. The buffer is intended for isolation and column equilibration to prepare for DNA binding. MOPS provides buffering capacity, isopropanol assists in establishing correct resin conditions, and Triton minimizes contamination and helps with endotoxin removal. A high concentration of sodium chloride functions as an electrostatic interaction regulator between pDNA and anion-exchange resin.

Specifications

Component Name	CAS	Molecular Weight	Concentration
MOPS, Free Acid	1132-61-2	209.26	50 mM
Sodium Chloride	7647-14-5	58.44	750 mM
Triton X-100	9036-19-5	~625	0.15 % (v/v)
Isopropyl Alcohol	67-63-0	60.10	15 % (v/v)
Sodium Hydroxide	1310-73-2	40.0	For pH Adjustment
Water	7732-18-5	18.02	Rest

Documents

Safety Data Sheets (SDS)Document request form

Need a specific formulation?

We can manufacture custom buffers to your exact specifications.

Custom Reagent Builder

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Need a specific formulation?

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Need a specific formulation?

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Need a specific formulation?

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Product Description

Specifications

Documents

Product Citations

Related Products

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Need a specific formulation?

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Plasmid DNA Wash Buffer (pH 7.0)

Plasmid DNA Elution Buffer (pH 8.5)

Plasmid DNA Neutralization Buffer (pH 5.5)

Plasmid DNA Resuspension Buffer (pH 8.0)

Plasmid DNA Lysis Solution (pH > 11.0)

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?

Plasmid DNA Isolation Buffer (pH 7.0)

Need a Custom Formulation?

Need a Custom Formulation?