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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Scientist handling electrophoresis buffer solutions in a research laboratory
  1. Home
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  4. Electrophoresis Buffers

Electrophoresis Buffers

Electrophoresis buffers are critical to the successful separation and analysis of nucleic acids and proteins. These pH-stabilized solutions ensure consistent ionic strength and minimize variability, supporting sharp band resolution and accurate migration in gel-based techniques.

  • Maintains stable pH and conductivity for reproducible separation
  • Supports DNA, RNA, and protein workflows with trusted formulations like TAE, TBE, and SDS-containing buffers
  • Compatible with agarose and polyacrylamide gels across a range of molecular weight targets

Whether you're running routine DNA gels or high-resolution SDS-PAGE, our electrophoresis buffers deliver reliable performance batch after batch.

Electrophoresis Buffers

Showing 21–33 of 33 products

Bis-Tris Transfer Buffer (20X)

Bis-Tris Transfer Buffer (20X)

From: $120.00

Bovine Serum Albumin, BSA (5%, TBST)

Bovine Serum Albumin, BSA (5%, TBST)

From: $57.00

Bovine Serum Albumin, BSA (5%, TBS)

Bovine Serum Albumin, BSA (5%, TBS)

From: $57.00

Bovine Serum Albumin, BSA (3%, TBS)

Bovine Serum Albumin, BSA (3%, TBS)

From: $89.00

Bovine Serum Albumin, BSA (3%, TBST)

Bovine Serum Albumin, BSA (3%, TBST)

From: $89.00

Gelatin (10%)

Gelatin (10%)

From: $44.00

LDS-Sample Buffer (4X, Reducing)

LDS-Sample Buffer (4X, Reducing)

From: $37.00

LDS-Sample Buffer (4X, Non Reducing)

LDS-Sample Buffer (4X, Non Reducing)

From: $37.00

Tris-Tricine-SDS Running Buffer (10X, Cathode Buffer)

Tris-Tricine-SDS Running Buffer (10X, Cathode Buffer)

From: $60.00

Tris-Tricine-SDS Running Buffer (10X, Anode Buffer)

Tris-Tricine-SDS Running Buffer (10X, Anode Buffer)

From: $55.00

Bovine Serum Albumin, BSA (3% in PBST)

Bovine Serum Albumin, BSA (3% in PBST)

From: $89.00

Bovine Serum Albumin (5%, in PBST)

Bovine Serum Albumin (5%, in PBST)

From: $57.00

Bovine Serum Albumin, BSA (5%, PBS)

Bovine Serum Albumin, BSA (5%, PBS)

From: $57.00

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FAQs

Electrophoresis buffers create the ionic environment needed for the migration of charged molecules, such as DNA, RNA, or proteins, through a gel matrix during electrophoresis.

TAE (Tris-acetate-EDTA) offers faster migration and is ideal for downstream gel extraction or enzymatic applications. TBE (Tris-borate-EDTA) provides better resolution for small fragments and maintains buffering capacity over longer runs.

Buffer choice depends on the type of molecule (DNA, RNA, protein), gel type, desired resolution, and compatibility with downstream recovery or enzymatic steps.