The 10X Tris-Tricine-SDS Anode Running Buffer is an anode, or positive electrode buffer in a gel electrophoresis system, facilitating the predictable flow of ions into a stable, buffered environment. The buffer contains only tris as a buffer, which allows the formation of an ion gradient for effective stacking. The buffer effectively stabilizes pH and current flow through the gel. The product is provided as a 10X concentrate, with a pH of 8.9 when diluted to 1X for use.