Tricine [N‑tris(hydroxymethyl)methylglycine] is a zwitterionic Good’s buffer widely employed in biochemical and molecular biology research. With an effective buffering range from pH 7.4 to 8.8, it provides stable near‑neutral conditions for diverse analytical and preparative techniques. This 0.2 M Tricine buffer is particularly valuable in SDS‑PAGE systems for resolving small‑ to mid‑sized peptides ranging from 1 to 100 kDa. In addition, it supports applications including capillary zone electrophoresis, ion‑exchange chromatography, HPLC, and cell resuspension, where consistent pH regulation enhances precision and reproducibility.