Tricine
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Tricine Buffer (0.2 M, pH 6.5)
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Tricine Buffer (0.2 M, pH 8.0)
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Tricine Buffer (0.2 M, pH 7.4)
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Tricine buffer (N-tris(Hydroxymethyl)methylglycine) is a versatile Good’s buffer from the Tris family, widely used in biochemistry and molecular biology. Known for its strong buffering capacity in electrophoresis and chromatography, Tricine is the preferred SDS-PAGE buffer for separating low molecular weight polypeptides (1–100 kDa). It also plays a key role in capillary zone electrophoresis, HPLC, ion exchange chromatography, and cell resuspension applications.
Whether you're working with protein separation, high-resolution chromatography, or molecular biology workflows, Tricine buffer offers exceptional stability and precision for high-quality results.

Showing 1–3 of 3 products

From: $44.00

From: $44.00

From: $44.00
Tricine has a lower ion mobility than glycine, providing better resolution for small polypeptides (1–100 kDa) in SDS-PAGE compared to traditional Tris-glycine buffers.
While Tris-glycine is commonly used for standard protein electrophoresis, Tricine is superior for separating smaller proteins and peptides due to its enhanced resolving power.
Yes! Tricine is frequently used in capillary zone electrophoresis, high-performance liquid chromatography (HPLC), and ion exchange chromatography due to its buffering efficiency and compatibility with protein and peptide analysis.