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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Lab technician preparing balanced salt solutions for cell culture
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Balanced Salt

Balanced Salt Solutions provide an isotonic environment for cells and tissues in culture, maintaining proper osmotic balance and pH during experiments.

  • Maintain osmotic balance and ionic strength
  • Support cell viability in vitro
  • Compatible with various cell types and tissues
  • Often used as a diluent or wash solution

Commonly used in cell culture, tissue preparation, and biochemical assays where maintaining physiological conditions is critical.

Balanced Salt

Showing 21–22 of 22 products

Hank's Balanced Salt Solution, HBSS (without Ca, Mg & Phenol Red)

Hank's Balanced Salt Solution, HBSS (without Ca, Mg & Phenol Red)

From: $47.00

Hank's Balanced Salt Solution, HBSS (10X, with Phenol Red)

Hank's Balanced Salt Solution, HBSS (10X, with Phenol Red)

From: $73.00

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FAQs

Products & Formulation

They are used to maintain cell viability and physiological conditions during experiments.

Yes, they are broadly compatible but check specific formulations for sensitive cell lines.