HBSS without Calcium, Magnesium, and Phenol Red is a balanced salt solution formulated to maintain osmotic balance and pH while omitting divalent cations that can interfere with cell dissociation. The absence of phenol red also makes this formulation suitable for applications sensitive to dye interference, such as fluorescence-based assays. Its calcium- and magnesium-free composition helps prevent premature re-adhesion of cells during trypsinization and other enzymatic dissociation steps.