This RIPA Buffer, balanced at pH 8.0, provides rapid and comprehensive lysis of mammalian cells and tissues for protein extraction. Designed to solubilize proteins from cytoplasmic, membrane‑bound, and nuclear fractions, it ensures efficient recovery of both structural and regulatory proteins. The optimized pH environment enhances compatibility with a wide range of downstream applications such as SDS‑PAGE, Western blotting, ELISA, and immunoprecipitation. Its strong detergent system promotes complete membrane disruption while preserving target antigen integrity.