The NP‑40 (2X) Lysis Buffer is a concentrated formulation designed for convenient dilution or use in applications requiring stronger detergent action. It relies on the non‑ionic surfactant NP‑40 to solubilize cellular membranes and release proteins in a controlled manner suitable for downstream analysis. This versatile reagent facilitates the efficient preparation of cell lysates for Western blotting, co‑immunoprecipitation, and enzymatic assays. The 2X concentration offers greater flexibility in adjusting lysis strength, allowing optimal recovery of cytoplasmic and partially nuclear proteins without excessive disruption of cellular architecture.