MES Buffer [2-(N-morpholino)ethanesulfonic acid] is a zwitterionic buffering agent commonly used in biological and biochemical research. It offers reliable pH stabilization between 5.5 and 7.0 and dissolves readily in aqueous solutions. This buffer is often selected for bis-tris gel electrophoresis, protein purification, and chromatographic applications. Its low UV absorbance and non-coordinating nature make it ideal for metal ion studies and enzyme-based procedures requiring consistent pH conditions.