The HBS-N Buffer (HEPES Buffered Saline) is a variant of HEPES Buffered Saline, useful in SPR (Surface Plasmon Resonance) protocols. This reagent is formulated at a pH of 7.4 and can be used to prime in-unit pumps within SPR devices. The HBS-N solution is useful in maintaining biomolecular stability and for assessing binding characteristic and biochemical dynamics between analytes. This solution is formulated at a 1X concentration.