Skip to navigationSkip to contentSkip to footer
Boston BioProducts

Stay informed about our latest scientific innovations and product updates

By subscribing, you agree to our privacy policy and consent to receive updates

Have a Question?

+1 (508) 231-4777

Mon - Fri: 8am - 5pm

Contact Us
  • Buffers
  • Solutions
  • Media
  • Chemical Powders
  • Water
  • Bioprocessing
  • Custom Buffer Manufacturing
  • Custom Buffer Manufacturing
  • GMP Reagent Manufacturing
  • White-Label and Kitting
  • Custom Buffer Builder
  • Reagent Stability Studies
  • QC Test Builder
  • Quality Assurance
  • Our Facilities
  • Quality Control Testing
  • Documentation Request
  • Content Hub
  • Buffer Calculator
  • FAQs
  • Order Support
  • About Us
  • Contact Us
  • Careers
  • News
  • Events

© 2026 Boston BioProducts. All Rights Reserved.

Privacy PolicyTerms of ServiceSitemap
Boston BioProductsBoston BioProducts
Boston BioProductsBoston BioProducts
Your quote is empty.
My Cart
  • Products
    Product
    • Buffers
    • Solutions
    • Media
    • Chemical Powders
    • Water
    • Bioprocessing
    • Custom Buffer Manufacturing
    Buffers
    • Good's Buffers
    • Buffered Saline
    • Electrophoresis Buffers
    • Lysis Buffers
    • Standard Buffers
    • Hybridization Buffers
    • HPLC/FPLC Buffers
    • qPCR Buffers
    View all Buffers
    Solutions
    • Acid & Base
    • Balanced Salts
    • Metal
    • EDTA & EGTA
    • Antibiotics
    • Detergent
    • Acrylamide
    • Sugar
    • Fixative
    • Inhibitor Solutions
    • Enzyme
    • Dyes & Stains
    • Luminol & Oxidizing Agents
    View all Solutions

    Featured Products

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

    View product
    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

    View Product
    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

    View Product
  • Custom
    • Custom Buffer Manufacturing
    • GMP Reagent Manufacturing
    • White-Label and Kitting
    • Custom Buffer Builder
  • Services
    • Reagent Stability Studies
    • Quality Control Testing
    • QC Test Builder
  • Quality
    • Quality Assurance
    • Our Facilities
    • Quality Control Testing
      • Absorbance Testing
      • Bioburden Testing
      • Conductivity Testing
      • Density Testing
      • DNase Testing
      • Endotoxin Testing
      • Microbial Growth Testing
      • Osmolality Testing
      • pH Testing
      • Protease Testing
      • Refractive Index Testing
      • RNase Testing
      • Stability Testing
      • Sterility Testing
      • Viscosity Testing
      • Bubble Point (BP) Filter Integrity Testing
    • Documentation Request
  • Resources
    • Content Hub
      • Product Literature
      • Safety Data Sheets
      • Publications
      • Case Studies & Infographics
      • Protocols
      • Articles & Insights
    • Buffer Calculator
    • Applications
      • Molecular Biology
        • FPLC Protein Purification
        • SDS PAGE
        • HPLC Buffers
        • Plasmid Isolation
        • ELISA Buffers
        • Blood Lysis Buffers
      • Cell Culture
        • Cell Culture Buffers
        • Yeast and Bacteria Culture Media
        • Western Blotting Buffer
        • IHC Buffer
      • Diagnostics
        • Immuno Diagnostics
      • Bioproduction
        • Upstream Bioprocessing
        • Downstream Bioprocessing
    • FAQs
    • Order Support
  • Company
    • About Us
    • Contact Us
    • Careers
    • News
    • Events
My AccountContact Us
Your cart is empty.
HEPES Buffered Saline
  1. Home
  2. Products
  3. Buffers
  4. Buffered Saline
  5. HEPES Buffered Saline

HEPES Buffered Saline

HEPES Buffered Saline (HBS) is a versatile buffer solution designed to maintain stable, physiological pH (6.8–8.2) across a range of biological and biochemical applications. Containing HEPES as the primary buffering agent, HBS is less temperature-sensitive than Tris-based buffers, making it a reliable choice for sensitive experimental workflows.

HBS is ideal for:

Surface Plasmon Resonance (SPR) experiments due to low UV absorbance and excellent buffering stability

Cell culture and biochemical assays, where consistent pH is critical for protein and cellular function

Custom formulations with ions or additives tailored to specific research needs

  • Minimizes pH drift across temperature changes
  • Compatible with optical detection methods due to low UV absorbance
  • Supports protein integrity and cell viability in dynamic systems

Whether you're studying biomolecular interactions, culturing cells, or running high-sensitivity assays, HEPES Buffered Saline offers stability, flexibility, and performance.

HEPES Buffered Saline

Showing 1–11 of 11 products

5X Annexin Binding Buffer (for Flow Cytometry)

5X Annexin Binding Buffer (for Flow Cytometry)

From: $357.00

Live Cell Imaging Solution

Live Cell Imaging Solution

From: $46.00

HBS-EP+ Buffer (1X, pH 7.4)

HBS-EP+ Buffer (1X, pH 7.4)

From: $315.00

HBS-EP Buffer (1X, pH 7.4)

HBS-EP Buffer (1X, pH 7.4)

From: $315.00

HBS-P+ Buffer (1X, pH 7.4)

HBS-P+ Buffer (1X, pH 7.4)

From: $315.00

HBS-P+ Buffer (10X, pH 7.4)

HBS-P+ Buffer (10X, pH 7.4)

From: $378.00

HBS-N Buffer (1X, pH 7.4)

HBS-N Buffer (1X, pH 7.4)

From: $315.00

HBS-N Buffer (10X, pH 7.4)

HBS-N Buffer (10X, pH 7.4)

From: $378.00

HEPES Buffered Saline (5X, pH 7.4)

HEPES Buffered Saline (5X, pH 7.4)

From: $99.00

HEPES Buffered Saline (2X)

HEPES Buffered Saline (2X)

From: $44.00

HEPES Buffered Saline (2X, Low Endotoxin)

HEPES Buffered Saline (2X, Low Endotoxin)

From: $76.00

FAQs

HBS is less sensitive to temperature fluctuations than Tris, providing more consistent pH control in experiments where temperature changes may occur.

HBS has low UV absorbance and excellent buffering capacity, which helps prevent signal interference in surface plasmon resonance studies, ensuring accurate biomolecular interaction analysis.

Yes, HBS formulations can be adjusted with calcium, magnesium, or other additives to meet specific assay or cell culture requirements.