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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Scientist measuring Good's buffers for biological research in a lab
  1. Home
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  4. Good's Buffers

Good's Buffers

Achieve stable, reproducible results in your experiments with Good’s buffers, a trusted choice for biological and biochemical research. Originally developed by Norman E. Good in the 1960s, these zwitterionic compounds are designed to maintain precise pH control in aqueous solutions, particularly under physiological conditions. Explore our range of Good's buffers for:

  • Minimal biomolecular interaction ensures accuracy in sensitive assays
  • High chemical stability prevents degradation and variability
  • Low experimental interference maintains the integrity of cell culture, enzyme assays, and protein purification workflows

Whether you're optimizing experimental conditions or scaling up research, our Good’s buffers help you achieve reproducibility and precision every time.

Good's Buffers

Showing 21–40 of 55 products

PIPES Buffer (1 M, pH 7.5)

PIPES Buffer (1 M, pH 7.5)

From: $127.00

PIPES Buffer (1 M, pH 7.0)

PIPES Buffer (1 M, pH 7.0)

From: $127.00

PIPES Buffer (1 M, pH 6.8)

PIPES Buffer (1 M, pH 6.8)

From: $127.00

PIPES Buffer (0.5 M, pH 7.5)

PIPES Buffer (0.5 M, pH 7.5)

From: $44.00

PIPES Buffer (0.5 M, pH 7.0)

PIPES Buffer (0.5 M, pH 7.0)

From: $44.00

PIPES Buffer (0.5 M, pH 6.8)

PIPES Buffer (0.5 M, pH 6.8)

From: $44.00

MOPS Buffer (1 M, pH 7.5)

MOPS Buffer (1 M, pH 7.5)

From: $127.00

MOPS Buffer (1 M, pH 7.4)

MOPS Buffer (1 M, pH 7.4)

From: $127.00

MOPS Buffer (1 M, pH 7.0)

MOPS Buffer (1 M, pH 7.0)

From: $127.00

MES Buffer (1 M, pH 6.5)

MES Buffer (1 M, pH 6.5)

From: $127.00

MES Buffer (1 M, pH 6.0)

MES Buffer (1 M, pH 6.0)

From: $127.00

MES Buffer (1 M, pH 5.5)

MES Buffer (1 M, pH 5.5)

From: $127.00

MES Buffer (1 M, pH 5.0)

MES Buffer (1 M, pH 5.0)

From: $127.00

MES Buffer (0.5 M, pH 6.5)

MES Buffer (0.5 M, pH 6.5)

From: $64.00

MES Buffer (0.5 M, pH 6.0)

MES Buffer (0.5 M, pH 6.0)

From: $64.00

MES Buffer (0.5 M, pH 5.5)

MES Buffer (0.5 M, pH 5.5)

From: $64.00

HEPPS Buffer (0.2 M, pH 8.0)

HEPPS Buffer (0.2 M, pH 8.0)

From: $82.00

HEPES Buffer (1 M, pH 7.6)

HEPES Buffer (1 M, pH 7.6)

From: $127.00

HEPES Buffer (1 M, pH 7.5)

HEPES Buffer (1 M, pH 7.5)

From: $87.00

HEPES Buffer (1 M, pH 7.4)

HEPES Buffer (1 M, pH 7.4)

From: $127.00

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FAQs

Good’s buffers are a set of zwitterionic compounds designed to maintain stable pH in biochemical and biological research. They are widely used in cell culture, enzyme assays, and protein purification due to their high stability and minimal reactivity with biomolecules.

Common Good’s buffers include HEPES, MES, MOPS, PIPES, Tris, and Tricine. Each buffer is designed for specific pH ranges and applications, ensuring optimal performance in biological and biochemical experiments.

The selection depends on the required pH range and application. For example, HEPES is ideal for physiological conditions, MES is used in protein purification, and Tricine is optimal for SDS-PAGE separation of low molecular weight proteins.