The 1M HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a zwitterionic buffering agent widely used in in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This HEPES formulation is prepared in WFI-Quality water at a pH of 7.5, and is ideal for applications within Bioprocessing.