Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution and it functions as an efficient pH regulator, maintaining physiological pH of 7.2 to 7.6, while influencing the osmotic pressure via balanced salt content. This buffer supports several applications, including cell culture, immunology, and molecular biology. By adjusting concentrations of phosphate ions and sodium ions, buffering capacity tailored to specific needs can be achieved.