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Tris-Based
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Tris-Based

Tris-based buffer is a widely used buffering agent in biological and biochemical research. It maintains a stable pH in the range of 7.0 to 9.0, making it ideal for various laboratory applications, such as gel electrophoresis, resuspension, and enzymatic techniques.

  • Provides consistent pH conditions, essential for enzymatic reactions and nucleic acid stability
  • Pre-formulated and easily diluted to reduce setup time
  • Minimizes enzyme inhibition and degradation, helping preserve sample integrity

Whether you're working on DNA extraction, protein analysis, or cell culture experiments, our Tris-based buffers ensure reliable performance and reproducible results.

Tris-Based

Showing 1–14 of 14 products

Glucose-Tris EDTA Buffer, GTE (Nuclease Free)

Glucose-Tris EDTA Buffer, GTE (Nuclease Free)

From: $72.00

DNA/RNA Storage Buffer (pH 8.0, Nuclease Free)

DNA/RNA Storage Buffer (pH 8.0, Nuclease Free)

From: $35.00

Tris-EDTA Buffer, TE (pH 8.0, Nuclease Free)

Tris-EDTA Buffer, TE (pH 8.0, Nuclease Free)

From: $26.00

Tris-EDTA Buffer, TE (20X, pH 7.4, Nuclease Free)

Tris-EDTA Buffer, TE (20X, pH 7.4, Nuclease Free)

From: $31.00

Tris-Acetate-EDTA Buffer, TAE (50X)

Tris-Acetate-EDTA Buffer, TAE (50X)

From: $64.00

Tris-Acetate-EDTA Buffer, TAE (10X)

Tris-Acetate-EDTA Buffer, TAE (10X)

From: $70.00

Tris-Acetate Buffer (0.2 M, pH 7.8)

Tris-Acetate Buffer (0.2 M, pH 7.8)

From: $57.00

Tris-Acetate Buffer (0.2 M, pH 7.4)

Tris-Acetate Buffer (0.2 M, pH 7.4)

From: $57.00

Tris-Acetate Buffer (0.2 M, pH 7.0)

Tris-Acetate Buffer (0.2 M, pH 7.0)

From: $57.00

Tris-EDTA Buffer, TE (pH 8.0)

Tris-EDTA Buffer, TE (pH 8.0)

From: $25.00

Tris-EDTA Buffer, TE (pH 7.4)

Tris-EDTA Buffer, TE (pH 7.4)

From: $37.00

Tris-EDTA Buffer, TE (20X, pH 8.0)

Tris-EDTA Buffer, TE (20X, pH 8.0)

From: $31.00

Tris-EDTA Buffer, TE (pH 8.0, Low Endotoxin)

Tris-EDTA Buffer, TE (pH 8.0, Low Endotoxin)

From: $51.00

Tris-Borate-EDTA Buffer, TBE (10X)

Tris-Borate-EDTA Buffer, TBE (10X)

From: $95.00

FAQs

Tris-based buffers are used in electrophoresis, nucleic acid extraction, protein purification, western blotting, PCR, and cell culture.

Tris is compatible with many enzymes and biomolecules, but its buffering capacity can be temperature-sensitive and should be tested for specific applications.

Yes, Tris buffer pH is temperature-dependent and may shift slightly with changes in temperature, which should be considered for sensitive experiments.

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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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