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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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  1. Products
  2. Buffers
  3. Electrophoresis Buffers
  4. Blocking
  5. Casein (3%, TBS)
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Product Description

The Casein Buffer preserves enzyme stability during reactions, minimizes non-specific binding in immunoassays, and serves as a protein standard in a multitude of applications. When combined with a buffering agent TBS, casein helps maintain the structural stability of other proteins, protecting them from degradation at physiological pH. Maintaining the pH of an experiment with a casein buffer ensures uniform outcomes in a variety of biochemical and diagnostic processes.

Specifications

Component NameCASMolecular WeightConcentration
Casein9000-71-9N/A0.03
Tris-Base / Tris-HCl77-86-1 / 1185-53-1121.14 / 157.6050 mM
Sodium Chloride7647-14-558.44150 mM
Water7732-18-518.02Rest

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Casein (3%, PBS)

Casein (3%, PBS)

IBB-120
Bovine Serum Albumin, BSA (3%, TBS)

Bovine Serum Albumin, BSA (3%, TBS)

IBB-111
Tris Buffered Saline, TBS (20X)

Tris Buffered Saline, TBS (20X)

BM-300X
Bovine Serum Albumin, BSA (5%, TBS)

Bovine Serum Albumin, BSA (5%, TBS)

IBB-187
Tris Buffered Saline, TBS (10X, pH 7.4)

Tris Buffered Saline, TBS (10X, pH 7.4)

BM-300
Casein (3%, PBS)

Casein (3%, PBS)

IBB-120
Bovine Serum Albumin, BSA (3%, TBS)

Bovine Serum Albumin, BSA (3%, TBS)

IBB-111
Tris Buffered Saline, TBS (20X)

Tris Buffered Saline, TBS (20X)

BM-300X
Bovine Serum Albumin, BSA (5%, TBS)

Bovine Serum Albumin, BSA (5%, TBS)

IBB-187
Tris Buffered Saline, TBS (10X, pH 7.4)

Tris Buffered Saline, TBS (10X, pH 7.4)

BM-300
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Casein (3%, TBS)

CAT#: IBB-130
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  • Useful in cell culture, biochemistry, and molecular biology techniques
  • Commonly used in blocking steps within immunoassay such as Western blotting, ELISA, and within immunohistochemistry
  • Ready-to-use solution tested for pH

Need a Custom Formulation?

Modify concentration, pH, packaging, or quality grade.

We manufacture to your exact specifications.

Custom Reagent Builder
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Need a Custom Formulation?

Modify concentration, pH, packaging, or quality grade.

We manufacture to your exact specifications.

Custom Reagent Builder
Questions?
Call us at+1 (508) 231-4777
We Accept
  1. Products
  2. Buffers
  3. Electrophoresis Buffers
  4. Blocking
  5. Casein (3%, TBS)
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Casein (3%, TBS)

CAT#: IBB-130
Loading...
  • Useful in cell culture, biochemistry, and molecular biology techniques
  • Commonly used in blocking steps within immunoassay such as Western blotting, ELISA, and within immunohistochemistry
  • Ready-to-use solution tested for pH

Need a Custom Formulation?

Modify concentration, pH, packaging, or quality grade.

We manufacture to your exact specifications.

Custom Reagent Builder
Loading...
Questions?Call us at+1 (508) 231-4777
We Accept

Need a Custom Formulation?

Modify concentration, pH, packaging, or quality grade.

We manufacture to your exact specifications.

Custom Reagent Builder
Custom Size
CAT#: IBB-130-500ml

Total (USD)

$76.00

Custom Size
CAT#: IBB-130-500ml

Total (USD)

$76.00