The 2X RIPA Buffer is used for rapid, efficient cell lysis and solubilization of proteins from adherent and suspension cultured mammalian cells and tissue samples. This buffer is extremely useful for disruption of all proteins within cellular compartments, including nuclear, membrane, and mitochondrial proteins and is compatible with many downstream applications, such as SDS-PAGE, immunoprecipitation, Western blot, and ELISA assays. This product is made with Triton X and Glycerol.