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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Researcher preparing biological buffer solutions in a laboratory setting
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Buffers

Buffers are essential reagents formulated to maintain stable pH conditions in biological and biochemical systems. They help preserve the structure and function of proteins, nucleic acids, and other biomolecules during experimental procedures by resisting pH changes during reactions and manipulations.

  • Designed to maintain stable pH in specific ranges
  • Includes Good’s buffers, standard buffers, and specialized formulations
  • Low biological interference for reliable results
  • Available in ready‑to‑use or concentrated forms
  • Compatible with molecular biology, cell biology, and analytical workflows

Buffers are commonly used in enzyme assays, protein purification, electrophoresis, cell culture, and other workflows where precise pH control is critical.

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FAQs

Products & Formulation

Buffers help maintain a consistent pH environment during experiments, preventing shifts that can affect biomolecules and reactions.

This category includes Good’s buffers (e.g., MES, Bis Tris), standard buffers (e.g., Tris), and other specialized pH solutions.

Yes, because enzymes are often sensitive to pH, choosing the correct buffer range helps ensure optimal activity.

Many buffers are supplied ready to use; others are concentrated and require dilution according to protocol.