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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Lab technician preparing buffered saline solution with laboratory glassware
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Buffered Saline

Saline buffers are essential solutions used in biological and biochemical research to maintain pH stability and mimic physiological ionic conditions. They typically contain sodium chloride (NaCl) and a buffering agent like phosphate or Tris, offering both ionic strength and pH control critical for maintaining biomolecule integrity.

Common types include:

Phosphate-buffered saline (PBS): Ideal for cell culture, immunoassays, and washing steps

Tris-buffered saline (TBS): Widely used in western blotting, ELISA, and general molecular biology workflows

  • Maintains osmotic balance in sensitive biological systems
  • Supports protein and nucleic acid stability during processing
  • Compatible with a wide range of lab protocols and techniques

Whether you're performing cell-based assays, protein detection, or molecular biology workflows, saline buffers offer reliable performance and reproducibility across applications.

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FAQs

A saline buffer is a solution containing salt (like NaCl) and a buffering agent (such as phosphate or Tris) used to maintain stable pH and ionic conditions in biological experiments.

PBS (Phosphate-Buffered Saline) is commonly used in cell culture and immunoassays, while TBS (Tris-Buffered Saline) is preferred in protein-based assays like western blotting and ELISA due to its compatibility with antibody-based detection systems.

Saline buffers maintain physiological pH and ionic strength, preventing denaturation or degradation of proteins, nucleic acids, and other biomolecules during experiments.