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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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CAPS
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CAPS

CAPS (3-cyclohexylamino-1-propanesulfonic acid) is a zwitterionic buffer used in biological and biochemical research to maintain stable pH in strongly alkaline systems. It is especially useful for high-pH applications where standard near-neutral buffers are not effective.

  • pKa (25 °C): ~10.4
  • Effective buffering range: pH 9.7–11.1
  • Highly soluble in water
  • Low UV absorbance
  • Minimal interaction with proteins and enzymes

CAPS is commonly used in protein research, high-pH enzyme assays, Western blot transfer buffers, and other applications requiring reliable buffering at alkaline pH.

CAPS

Showing 1–2 of 2 products

CAPS Buffer (0.5 M, pH 11.0)

CAPS Buffer (0.5 M, pH 11.0)

From: $101.00

CAPS Buffer (0.5 M, pH 10.0)

CAPS Buffer (0.5 M, pH 10.0)

From: $101.00

FAQs

Products & Formulation

CAPS buffers effectively in the alkaline range from about pH 9.7 to 11.1, with optimal buffering near pH 10.4.

Use CAPS when you need stable buffering in strongly alkaline conditions — for example, in high-pH enzyme assays, protein electrophoresis transfer systems, or other biochemical applications that require pH well above neutral.