What pH range does Bis-Tris buffer?

Bis-Tris buffers effectively between pH 5.8 and 7.2, making it suitable for many biological systems.

What applications is Bis-Tris used for?

It is typically used in protein and nucleic acid purification, biochemical assays, electrophoresis, and molecular biology workflows.

Bis-Tris Buffer

Bis-Tris is a zwitterionic Good’s buffer commonly used in biochemical and molecular biology research. It helps maintain a stable pH environment in the near-neutral range with minimal interaction with biomolecules.

Effective buffering range: pH 5.8–7.2
pH useful for protein and nucleic acid work
Zwitterionic, low biological interference
Good water solubility
Helps support sensitive biochemical assays

Bis-Tris is commonly used in protein research, purification, electrophoresis and other applications requiring stable buffering near pH 6–7.

Bis-Tris

Bis-Tris

Bis-Tris Buffer (0.2 M, pH 6.5)

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