What is the buffering range of Bicine?

Bicine buffers effectively between pH 7.6 and 9.0, with optimal performance near its pKa of 8.35 at 25°C.

What applications is Bicine used for?

Bicine is typically used in biochemical and molecular biology applications that require stable buffering in the slightly alkaline range.

Bicine Buffers

Bicine is a zwitterionic Good’s buffer used in biological and biochemical research. It is effective in the slightly alkaline pH range and is designed to provide stable buffering with minimal interference in enzymatic and protein-based systems.

pKa (25°C): ~8.35
Effective buffering range: pH 7.6–9.0
Highly soluble in water
Low UV absorbance
Minimal interaction with biological molecules

Bicine is commonly used in enzyme assays, protein purification, electrophoresis systems, and other applications requiring reliable buffering near pH 8.